NRPS-PKS -- A Knowledge-based Resource for Analysis of NRPS/PKS Megasynthases
What you can do:
Analyze large multi-enzymatic, multi-domain megasynthases that are involved in the biosynthesis of pharmaceutically important natural products such as cyclosporin, rifamycin and erythromycin.
Highlights:
- Apart from correct identification of NRPS and PKS domains, NRPS-PKS can also predict specificities of adenylation and acyltransferase domains with reasonably high accuracy, making it a valuable resource for identification of natural products biosynthesized by NRPS/PKS gene clusters found in newly sequenced genomes.
- NRPS-PKS is a unique resource which provides a user-friendly interface for correlating chemical structures of natural products with the domains and modules in the corresponding nonribosomal peptide synthetases or polyketide synthases.
- It also provides guidelines for domain/module swapping as well as site-directed mutagenesis experiments to engineer biosynthesis of novel natural products.
Keywords:
- megasynthases
- nonribosomal peptide synthetases
- NRPS
- polyketide synthases
- protein domains
- adenylation domains
- acyltransferase domains
- enzymes
- cyclosporin biosynthesis
- rifamycin biosynthesis
- erythromycin biosynthesis
- antibiotics biosynthesis
Literature & Tutorials:
PubMed Link: NRPS-PKS
This record last updated: 05-11-2005