SNPbox -- Web-based High-throughput Primer Design from Gene to Genome
What you can do:
Automatically design PCR primers for large-scale amplification and sequencing projects aimed at constructing single nucleotide polymorphisms (SNPs) maps.
Highlights:
- Based on Primer3 algorithm, SNPbox can be used for genomic sequences up to 200 kb.
- The server also hosts the data of SNPbox analysis of all Ensembl exons.
- The data of this genome-wide SNPbox application can be visualized in Ensembl's ContigView through a DAS (Distributed Annotation System) annotation server.
- For its 2005 release, SNPbox automates and standardizes the generation of PCR primers and is used in the strategy for constructing single nucleotide polymorphisms (SNPs) maps.
- Primer conditions were chosen such that PCR amplifications are uniform for each PCR amplicon facilitating the use of high-throughput genetic platforms.
- SNPbox can also be used for the design of primer sets for mutation analysis, STR marker genotyping and microarray oligo design.
Keywords:
- PCR primers
- PCR primer design tool
- SNP genotyping primer design tool
- mutation analysis primers
- microarray oligo design tool
- Short Tandem Repeat markers (STR) design tool
Literature & Tutorials:
PubMed Link to the original article (2004): SNPbox -- Web-based High-throughput Primer Design from Gene to Genome
PubMed Link to 2005 update: SNPbox: a modular software package for large-scale primer design
PubMed Link to 2005 update: SNPbox: a modular software package for large-scale primer design
This record last updated: 08-14-2006